Prompt

A labeled diagram would depict: 5’ Cap: 7-methylguanosine linked via 5’-5’ triphosphate to RNA, with associated enzymes (triphosphatase, guanylyltransferase, methyltransferase). 3’ Processing: Pre-mRNA showing AAUAAA and GU-rich regions, cleavage by CPSF/CstF, and poly-A tail addition by PAP. Splicing: Spliceosome (snRNPs) assembling on intron, lariat formation, and exon ligation. Each step underscores the precision required for mRNA stability and translatability. Aberrations in these processes highlight the delicate balance between mRNA synthesis and degradation, directly influencing gene expression outcomes. In a handwritten with a pencil, scanned, notebook paper.

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